bs-3395R [Primary Antibody]
SGK1 (Ser78) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SGK1 Ser78

Modification Site: Ser78

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6446

Swiss Prot: O00141

Source: KLH conjugated synthetic phosphopeptide derived from human SGK1 around the phosphorylation site of Ser78

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 49


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney labeled with Anti-Phospho-SGK1 (Ser78) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1:A431 lysates Lane 2: Mouse stomach probed with Rabbit Anti-SGK1 (Ser78) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Mouse muscle lysates probed with Rabbit Anti-SGK1 (Ser78) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with Phospho-SGK1 (Ser78) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.