DATASHEET
Host:
Rabbit
Target Protein:
AIF1/Iba1
Immunogen Range:
6-36/147
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
199
Swiss Prot:
P55008
Source:
KLH conjugated synthetic peptide derived from human AIF1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with
AIF1/Iba1 Polyclonal Antibody, Unconjugated (bs-34006R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Human HL-60 cell lysates; Lane 2: Human U937 cell lysates probed with AIF1/Iba1 Polyclonal Antibody, Unconjugated (bs-34006R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.