bs-34012R [Primary Antibody]
Bcl-2 Polyclonal Antibody
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Host: Rabbit

Target Protein: Bcl-2

Immunogen Range: 21-120/236


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 596

Swiss Prot: P49950

Source: KLH conjugated synthetic peptide derived from human Bcl-2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 26


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang Wei. et al. C1QTNF6 regulates cell proliferation and apoptosis of NSCLC in vitro and in vivo. Bioscience Rep. 2021 Jan;41(1):BSR20201541Read more>>
  • Leiming Sun. et al. Isoliquiritigenin attenuates acute renal injury through suppressing oxidative stress, fibrosis and JAK2/STAT3 pathway in streptozotocin-induced diabetic rats. Bioengineered. 2021;12(2):11188-112Read more>>
  • Pengcheng Zhou. et al. Tiao-bu-fei-shen formula promotes downregulation of the caveolin 1-p38 mapk signaling pathway in COPD - Associated tracheobronchomalacia cell model. J ETHNOPHARMACOL. J Ethnopharmacol. 2022 Apr;:115256Read more>>
  • Cai Jiumei. et al. Silencing lncRNA 93358 Inhibits the Apoptosis of Myocardial Cells in Myocardial Infarction Rats by Inducing the Expression of SLC8A1. BIOMED RES INT. 2022;2022:1138709Read more>>
  • Cheng He. et al. Crosstalk of renal cell carcinoma cells and tumor-associated macrophages aggravates tumor progression by modulating muscleblind-like protein 2/B-cell lymphoma 2/beclin 1-mediated autophagy. CYTOTHERAPY. 2022 OcRead more>>
  • Yu Zhou. et al. Human amniotic mesenchymal stromal cell-derived exosomes promote neuronal function by inhibiting excessive apoptosis in a hypoxia/ischemia-induced cerebral palsy model: A preclinical study. BIOMED PHARMACOTHER. 2024 Apr;173:116321Read more>>
  • Jian Shi. et al. Mechanistic elucidation of QiJu-DiHuang Wan in management of age-related dry eye through metabolomics and network pharmacology. PHYTOMEDICINE. 2024 Sep;132:155884Read more>>
VALIDATION IMAGES

Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lymph node lysates; Lane 3: Rat Lymph node lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human Siha cell lysates; Lane 6: Human Jurkat cell lysates; Lane 7: Human A431 cell lysates; Lane 8: Rat Bone lysates; Lane 9: Mouse Bone lysates; Lane 10: Human A549 cell lysates; Lane 11: Human SH-SY5Y cell lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-34012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with Bcl-2 polyclonal antibody, unconjugated (bs-34012R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.