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Host: Rabbit
Target Protein: ZO-1
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7082
Swiss Prot: Q07157
Source: KLH conjugated synthetic peptide derived from human ZO-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
Flow-Cyt(Flow-Cyt=1μg/Test)
ICC/IF(ICC/IF=1:50-200)
Predicted Molecular Weight: 191
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with ZO-1 polyclonal antibody, unconjugated (bs-34023R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.