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Host: Rabbit
Target Protein: VEGF
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7422
Swiss Prot: P15692
Source: KLH conjugated synthetic peptide derived from human VEGF
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100uL
Concentration: 1ug/ul
Applications:
WB(1:500-2000)
ELISA(1:5000-10000)
Predicted Molecular Weight: 24
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
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Lane 1: Human Huvec cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human THP-1 cell lysates ; Lane 5: Human A431 cell lysates;Lane 6: Human U87MG cell lysates; Lane 7: Human Hela cell lysates; Lane 8: Human Jurkat cell lysates; Lane 9: Human MCF-7 cell lysates probed with VEGF Polyclonal Antibody, Unconjugated (bs-34032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with VEGF165 polyclonal antibody, unconjugated (bs-34032R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.