bs-34063R [Primary Antibody]
CD105 Polyclonal Antibody
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Host: Rabbit

Target Protein: CD105

Immunogen Range: 351-450/625


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2022

Swiss Prot: P17813

Source: KLH conjugated synthetic peptide derived from human CD105

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Predicted Molecular Weight: 70


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jiaqiang Deng. et al. Curcumin Alleviates the Senescence of Canine Bone Marrow Mesenchymal Stem Cells during In Vitro Expansion by Activating the Autophagy Pathway. Int J Mol Sci. 2021 Jan;22(21):11356Read more>>
VALIDATION IMAGES

Lane 1: Human Huvec cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human U87MG cell lysates probed with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Serous ovarian cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.