bs-3464R-Biotin [Conjugated Primary Antibody]
ATG1(Ser556) Polyclonal Antibody, Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ATG1 Ser556

Modification Site: Ser556

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8408

Swiss Prot: O75385

Source: KLH conjugated synthetic phosphopeptide derived from human ATG1 around the phosphorylation site of Ser556

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 116


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Pandey, Aseem, et al. "Global Reprogramming of Host Kinase Signaling in Response to Fungal Infection." Cell Host & Microbe 21.5 (2017): 637-649.Read more>>
  • Peng Lin. et al. Polystyrene nanoplastics exacerbate lipopolysaccharide-induced myocardial fibrosis and autophagy in mice via ROS/TGF-1/Smad. TOXICOLOGY. 2022 Oct;480:153338Read more>>
  • Ramona DAmico. et al. Complex Interplay between Autophagy and Oxidative Stress in the Development of Endometriosis. ANTIOXIDANTS-BASEL. 2022 Dec;11(12):2484Read more>>
  • Chunli Yang. et al. BMAL1 involved in autophagy and injury of thoracic aortic endothelial cells of rats induced by intermittent heat stress through the AMPK/mTOR/ULK1 pathway. BIOCHEM BIOPH RES CO. 2023 Jun;661:34Read more>>
VALIDATION IMAGES