bs-3466R [Primary Antibody]
VASP(Ser239) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VASP Ser239

Specificity: This phosphorylation site is homologous to that of Ser235 in Mouse and Ser236 in Rat.

Modification Site: Ser239

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7408

Swiss Prot: P50552

Source: KLH conjugated synthetic phosphopeptide derived from human VASP around the phosphorylation site of Ser239

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 40


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse thymus lysates probed with VASP(Ser239) Polyclonal Antibody, Unconjugated (bs-3466R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.