Host: Rabbit
Target Protein: cdc25C Thr48
Modification Site: Thr48
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 995
Swiss Prot: P30307
Source: KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Thr48
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 53
Cross Reactive Species:
Human
Mouse
Rat
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Yin, H., et al. "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." Oncotarget (2016).Read more>>
- Guo, Hongrui, et al. "Dietary NiCl2 causes cell cycle arrest in the broiler's kidney." Oncotarget. (2015) 6.34:35964-77.Read more>>
- Fang, Jing, et al. "The Molecular Mechanisms of Protective Role of Se on the G2/M Phase Arrest of Jejunum Caused by AFB1." Biological Trace Element Research (2017): 1-12.Read more>>
Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Phospho-cdc25C (Thr48) Polyclonal Antibody, Unconjugated (bs-3482R) at 1:200 followed by conjugation to the secondary antibody and DAB
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-cdc25C (Thr48)) polyclonal Antibody, Unconjugated (bs-3482R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.