bs-3511R [Primary Antibody]
CD64/IGFR1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD64/IGFR1

Immunogen Range: 101-200/374


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2209

Swiss Prot: P12314

Source: KLH conjugated synthetic peptide derived from human CD64/IGFR1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
ICC(1:100-500)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Venosa Alessandro. et al. Role of CCR2+ Myeloid Cells in Inflammation Responses Driven by Expression of a Surfactant Protein-C Mutant in the Alveolar Epithelium. Front Immunol. 2021 Apr;12:1348Read more>>
  • Laura Lopez-Sanz. et al. Fc receptor activation mediates vascular inflammation and abdominal aortic aneurysm development. Clin Transl Med. 2021 Jul;11(7):e463Read more>>
  • Yin Zhuang. et al. Exosomes Secreted by Nucleus Pulposus Stem Cells Derived From Degenerative Intervertebral Disc Exacerbate Annulus Fibrosus Cell Degradation via Let-7b-5p. Front Mol Biosci. 2021; 8: 766115Read more>>
VALIDATION IMAGES

Lane 1: rat lung lysates Lane 2: human colon carcinoma lysates probed with Anti CD64/IGFR1/FCGR1A Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 41kD. Observed band size: 41kD.


Lane 1: U937 cell lysates probed with FCGR1A/CD64 Polyclonal Antibody, Unconjugated (bs-3511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Liver tissue lysates; Lane 4: Mouse Kidney tissue lysates ; Lane 5: Mouse Raw264.7 cell lysates ; Lane 6: Rat Spleen tissue lysates; Lane 7: Rat Liver tissue lysates; Lane 8: Rat Kidney tissue lysates; Lane 9: Human THP-1 cell lysates; Lane 10: Human HL60 cell lysates probed with FCGR1A/CD64 Polyclonal Antibody, Unconjugated (bs-3511R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.