bs-3513R [Primary Antibody]
Nur77 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: Nur77

Immunogen Range: 351-450/598


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3164

Swiss Prot: P22736

Source: KLH conjugated synthetic peptide derived from human Nur77/GFRP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 66


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti Nur77 Polyclonal Antibody (bs-3513R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


Sample: <br> Lane 1: Human Jurkat cell lysates<br> Lane 2: Human HeLa cell lysates<br> Primary: Anti-Nur77 (bs-3513R) at 1/1000 dilution<br> Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br> Predicted band size: 66 kDa<br> Observed band size: 73 kDa<br> Lot: AI11268723