bs-3551R [Primary Antibody]
RIPK3 Polyclonal Antibody
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Host: Rabbit

Target Protein: RIPK3

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11035

Source: KLH conjugated synthetic peptide derived from human RIPK3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains an N terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Over expression of RIP3 induces apoptosis and NF-kB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Luo, Yuhao, et al. "Lycorine induces programmed necrosis in the multiple myeloma cell line ARH-77." Tumor Biology (2014): 1-9.Read more>>
  • Yang B et al. Polychlorinated Biphenyl Quinone Promotes Macrophage-Derived Foam Cell Formation. Chem Res Toxicol. 2019 Nov 13.Read more>>
  • Yin Hongna. et al. Jia-Ji Electro-Acupuncture Improves Locomotor Function With Spinal Cord Injury by Regulation of Autophagy Flux and Inhibition of Necroptosis. Front Neurosci-Switz. 2020; 14: 616864Read more>>
  • Qin Yang. et al. Gypenoside XLIX protects against acute kidney injury by suppressing IGFBP7/IGF1R-mediated programmed cell death and inflammation. Phytomedicine. 2021 Mar;:153541Read more>>
  • Yin, Chunping. et al. Necrostatin-1 Against Sevoflurane-Induced Cognitive Dysfunction Involves Activation of BDNF/TrkB Pathway and Inhibition of Necroptosis in Aged Rats. Neurochem Res. 2022 Jan;:1-13Read more>>
  • Hu, Haoqiang. et al. Activation of necroptosis pathway in podocyte contributes to the pathogenesis of focal segmental glomerular sclerosis. CLIN EXP NEPHROL. 2022 Aug;:1-12Read more>>
  • Lei Lei. et al. Selenium Deficiency-Induced Oxidative Stress Causes Myocardial Injury in Calves by Activating Inflammation, Apoptosis, and Necroptosis. ANTIOXIDANTS-BASEL. 2023 Feb;12(2):229Read more>>
  • Shuang Wang. et al. Paricalcitol Ameliorates Acute Kidney Injury in Mice by Suppressing Oxidative Stress and Inflammation via Nrf2/HO-1 Signaling. INT J MOL SCI. 2023 Jan;24(2):969Read more>>
  • Xin Cao. et al. Alleviation of temporomandibular joint osteoarthritis by targeting RIPK1-mediated inflammatory signalling. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 AugRead more>>
  • Ke Mo. et al. Targeting hnRNPC suppresses thyroid follicular epithelial cell apoptosis and necroptosis through m6A-modified ATF4 in autoimmune thyroid disease. PHARMACOL RES. 2023 Sep;:106933Read more>>
  • Jiali Ye. et al. Polystyrene nanoplastics and cadmium co-exposure aggravated cardiomyocyte damage in mice by regulating PANoptosis pathway. ENVIRON POLLUT. 2024 Mar;:123713Read more>>
VALIDATION IMAGES

Lane 1:A549 lysates probed with Rabbit Anti-RIPK3 Polyclonal Antibody, Unconjugated (bs-3551R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Mouse spleen lysates probed with RIPK3 Polyclonal Antibody, Unconjugated (bs-3551R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Jurkat cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human Raji cell lysates probed with RIPK3 Polyclonal Antibody, Unconjugated (bs-3551R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.