bs-3570R
[Primary Antibody]
StAR Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: StAR
Immunogen Range: 101-200/285
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6770
Swiss Prot: P49675
Source: KLH conjugated synthetic peptide derived from human StAR
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 32
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Sheep
Predicted Cross Reactive Species:
Dog
Cow
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhao, Yong, et al. "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." Theriogenology (2016).Read more>>
- Shi, Lei, et al. "Effects of selenium on the proliferation, apoptosis and testosterone production of sheep Leydig cells in vitro." Theriogenology 93 (2017): 24-32.Read more>>
- Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
- Cook et al. Ethanol alters local cellular levels of (3α,5α)-3-hydroxypregnan-20-one (3α,5α-THP) independent of the adrenals in subcortical brain regions. (2014) Neuropsychopharmacology. 39:1978-87Read more>>
- Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology 313 (2016): 149-158.Read more>>
- Xiao L et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol. 2019 Jan 22. Read more>>
- Yang F et al. Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis. Brazilian Journal of Poultry Science.2019 Feb.Read more>>
- Xiaodan Li. et al. Effects of SPATA6 on proliferation, apoptosis and steroidogenesis of Hu sheep Leydig cells in?vitro. Theriogenology. 2021 May;166:9Read more>>
- Wenqian Xie. et al. Seasonal expressions of SF-1, StAR and P450scc in the scent glands of the muskrats (Ondatra zibethicus). J Steroid Biochem. 2020 Nov;204:105766Read more>>
- Junjie Hu. et al. Assessment of progesterone synthesis and its regulation role on dihydrotestosterone secretion in sheep epididymis. Gene. 2021 Jul;790:145699Read more>>
- Lu-ming Wu. et al. Dulaglutide, a long-acting GLP-1 receptor agonist, can improve hyperandrogenemia and ovarian function in DHEA-induced PCOS rats. Peptides. 2021 Nov;145:170624Read more>>
- Hua Yang. et al. SMAD2 regulates testicular development and testosterone synthesis in Hu sheep. Theriogenology. 2021 Oct;174:139Read more>>
- Ding Ziqiang. et al. Regulation of progesterone during follicular development by FSH and LH in sheep. ANIM REPROD. 2022 Jul;19Read more>>
- Chong Li. et al. The adiponectin receptor agonist, AdipoRon, promotes reproductive hormone secretion and gonadal development via the hypothalamic-pituitary-gonadal axis in chickens. POULTRY SCIENCE. 2022 Nov;:102319Read more>>
- Ozge Goktepe. et al. The effect of different doses of nonylphenol on the blood-testicular barrier integrity, hormone level, and DNA damage in the testes of rats. FOOD CHEM TOXICOL. 2023 Jul;177:113816Read more>>
- Wenjing Lu. et al. Seasonal changes of vitamin D3 and ovarian steroidogenesis in the wild ground squirrels (Citellus dauricus Brandt). J STEROID BIOCHEM. 2023 Nov;234:106385Read more>>
- Haoran Xu. et al. Melatonin Inhibits Testosterone Synthesis in Rooster Leydig Cells by Targeting CXCL14 through miR-7481-3p. INT J MOL SCI. 2023 Jan;24(23):16552Read more>>
- Hongwei Duan. et al. Melatonin promotes progesterone secretion in sheep luteal cells by regulating autophagy via the AMPK/mTOR pathway. THERIOGENOLOGY. 2023 NovRead more>>
- Wenjing Lu. et al. Vitamin D status alters genes involved in ovarian steroidogenesis in muskrat granulosa cells. BBA-MOL CELL BIOL L. 2024 May;1869:159469Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti StAR/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti StAR/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse ovary lysates L2 mouse brain lysates probed with Anti StAR/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 32kD. Observed band size:32kD.\n
Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Ovary lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Kidney lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Human K562cell lysates; Lane 6: Human THP-1 cell lysates; Lane 7: Human U937 cell lysates; Lane 8: Human HepG2 cell lysates probed with StAR Polyclonal Antibody, Unconjugated (bs-3570R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.