bs-3573R [Primary Antibody]
ADAMTS5 Polyclonal Antibody
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Host: Rabbit

Target Protein: ADAMTS5

Immunogen Range: 511-610/930


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11096

Swiss Prot: Q9UNA0

Source: KLH conjugated synthetic peptide derived from human ADAMTS5

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)
ELISA(1:5000-10000)

Predicted Molecular Weight: 102 kDa


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523.Read more>>
  • Liu Y et al. Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro. Oxid Med Cell Longev. 2019 Nov 6;2019:7189854.Read more>>
  • Yan L et al. Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity. Cell Biology and Toxicology. Read more>>
  • Kang X et al. Neuropeptide Y Acts Directly on Cartilage Homeostasis and Exacerbates Progression of Osteoarthritis through NPY2R. J Bone Miner Res. 2020 Feb 26.Read more>>
  • Kang X et al. Neuropeptide Y Acts Directly on Cartilage Homeostasis and Exacerbates Progression of Osteoarthritis through NPY2R. J Bone Miner Res. 2020 Feb 26.Read more>>
  • Weber AE et al. Modulation of Hedgehog Signaling by Kappa Opioids to Attenuate Osteoarthritis. Arthritis Rheumatol. 2020 Aug;72(8):1278-1288.Read more>>
  • Chen Guokun. et al. Circadian Rhythm Protein Bmal1 Modulates Cartilage Gene Expression in Temporomandibular Joint Osteoarthritis via the MAPK/ERK Pathway. Front Pharmacol. 2020 Sep;11:1402Read more>>
  • Luo Liwen. et al. Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes. Front Cell Dev Biol. 2021 Mar;9:482Read more>>
  • Jihang Dai. et al. Glabridin inhibits osteoarthritis development by protecting chondrocytes against oxidative stress, apoptosis and promoting mTOR mediated autophagy. Life Sci. 2021 Mar;268:118992Read more>>
  • Tao Yang. et al. Vitamin D3 protects articular cartilage by inhibiting the Wnt/?catenin signaling pathway. Exp Ther Med. 2020 Aug;20(2):1775-1781Read more>>
  • Quan Zhang. et al. Intra-articular injection of human umbilical cord mesenchymal stem cells ameliorates monosodium iodoacetate-induced osteoarthritis in rats by inhibiting cartilage degradation and inflammation. Bone Joint Res. 2021 Mar; 10(3): 226C236Read more>>
  • Yang, Jiashu. et al. m6A-mediated upregulation of AC008 promotes osteoarthritis progression through the miR-328-3p?AQP1/ANKH axis. Exp Mol Med. 2021 Nov;:1-12Read more>>
  • Wenhui Hu. et al. Farnesoid X receptor agonist attenuates subchondral bone osteoclast fusion and osteochondral pathologies of osteoarthritis via suppressing JNK1/2/NFATc1 pathway. Faseb J. 2022 Apr;36(4):e22243Read more>>
  • Fanqi Hu. et al. Identification of inflammatory regulation roles of thalidomide/ruxolitinib in nucleus pulposus and construction of polyelectrolyte nanocomplexes-impregnated injectable hydrogels for synergistic intervertebral disk degeneration treatment. Nano Today. 2022 Jun;44:101462Read more>>
  • Yang, Ke. et al. Comparisons between needle puncture and chondroitinase ABC to induce intervertebral disc degeneration in rabbits. EUR SPINE J. 2022 Jun;:1-13Read more>>
  • Yuhan Qu. et al. Chicoric acid attenuates tumor necrosis factor--induced inflammation and apoptosis via the Nrf2/HO-1, PI3K/AKT and NF-B signaling pathways in C28/I2 cells and ameliorates the progression of osteoarthritis in a rat model. INT IMMUNOPHARMACOL. 2022 Oct;111:109129Read more>>
  • Qun Lin. et al. Galangin ameliorates osteoarthritis progression by attenuating extracellular matrix degradation in chondrocytes via the activation of PRELP expression. EUR J PHARMACOL. 2022 Dec;936:175347Read more>>
  • Shen, Yue. et al. Hederagenin Suppresses Inflammation and Cartilage Degradation to Ameliorate the Progression of Osteoarthritis: An In vivo and In vitro Study. INFLAMMATION. 2022 Nov;:1-24Read more>>
  • Guoqing Li. et al. Teriparatide ameliorates articular cartilage degradation and aberrant subchondral bone remodeling in DMM mice. J ORTHOP TRANSL. 2023 Jan;38:241Read more>>
  • Lei Huang. et al. miR-199a-5p Reduces Chondrocyte Hypertrophy and Attenuates Osteoarthritis Progression via the Indian Hedgehog Signal Pathway. J CLIN MED. 2023 Jan;12(4):1313Read more>>
  • Qi Guo. et al. Isobavachalcone ameliorates the progression of osteoarthritis by suppressing NF-B signaling pathway. INT IMMUNOPHARMACOL. 2023 Jun;119:110102Read more>>
  • Li Guoqing. et al. An injectable liposome-anchored teriparatide incorporated gallic acid-grafted gelatin hydrogel for osteoarthritis treatment. NAT COMMUN. 2023 May;14(1):1-18Read more>>
  • Kuangyang Yang. et al. Identification of Andrographolide as a novel FABP4 inhibitor for osteoarthritis treatment. PHYTOMEDICINE. 2023 Sep;118:154939Read more>>
  • Qian Jiajia. et al. Swimming prevents cell death of chondrocytes via PI3K/AKT pathway in an experimental model. J ORTHOP SURG RES. 2023 Dec;18(1):1-9Read more>>
  • Fan Yang. et al. Abnormal mechanical stress induced chondrocyte senescence by YAP loss-mediated METTL3 upregulation. ORAL DIS. 2023 NovRead more>>
  • Qianhao Li. et al. Hypobaric hypoxia aggravates osteoarthritis via the alteration of the oxygen environment and bone remodeling in the subchondral zone. FASEB J. 2024 Apr;38(7):e23594Read more>>
  • Meng-Jia Li. et al. Biomechanism of abnormal stress on promoting osteoarthritis of temporomandibular joint through Piezo1 ion channel. J ORAL REHABIL. 2024 JuRead more>>
  • Fan Zhengrui. et al. Suppression of YAP Ameliorates Cartilage Degeneration in Ankle Osteoarthritis via Modulation of the Wnt/-Catenin Signaling Pathway. CALCIFIED TISSUE INT. 2024 Jul;:1-15Read more>>
  • Limeiting Wang. et al. TDP-43 ameliorates aging-related cartilage degradation through preventing chondrocyte senescence. EXP GERONTOL. 2024 Oct;195:112546Read more>>
VALIDATION IMAGES

Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human HeLa cell lysates probed with ADAMTS5 Polyclonal Antibody, Unconjugated (bs-3573R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.