bs-3576R [Primary Antibody]
HB-EGF Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HB-EGF

Immunogen Range: 51-150/208


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1839

Swiss Prot: Q99075

Source: KLH conjugated synthetic peptide derived from human HB-EGF/DTSF

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 21


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lebkuechner, Isabell, et al. "Heterogeneity of Notch signaling in astrocytes and the effects of GFAP and vimentin deficiency." Journal of Neurochemistry(2015).Read more>>
  • Lebkuechner et al. Heterogeneity of Notch signaling in astrocytes and the effects of GFAP and vimentin deficiency. (2015) J.Neurochem. 135:234-48Read more>>
  • Maximilian Strunzet al. Alveolar regeneration through a Krt8+ transitional stem cell state that persists in Human lung fibrosis. Nat Commun. 2020 Jul 16;11(1):3559.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-HB EGF Polyclonal Antibody, Unconjugated (bs-3576R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat kidney labeled with Anti-HB EGF Polyclonal Antibody, Unconjugated (bs-3576R) at 1:200 followed by conjugation to the secondary antibody and DAB staining