DATASHEET
Host:
Rabbit
Target Protein:
T1 gamma
Immunogen Range:
981-1127/1127
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
51592
Swiss Prot:
Q9UPN9
Source:
KLH conjugated synthetic peptide derived from human TIF1 gamma
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
Conjugation:
ALEXA FLUOR® 488
Excitation/ Emission:
490nm/525nm
Size:
100ul
Concentration:
1ug/ul
Applications:
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
124
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Molt4 cells(black) were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TIF1 gamma Polyclonal Antibody, A488 Conjugated (bs-3582R-A488) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).