bs-3589R-A750 [Conjugated Primary Antibody]
eNOS (Thr113) Polyclonal Antibody, ALEXA FLUOR® 750 Conjugated
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: eNOS Thr113

Specificity: This phosphorylation site is homologous across the listed species; this phosphorylation site does not exist in human due to a Thr-Ser substitution.

Modification Site: Thr113

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18127

Swiss Prot: P70313

Source: KLH conjugated synthetic phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser113

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.

Conjugation: ALEXA FLUOR® 750

Excitation/ Emission: 749nm/775nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 133


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yi Ren. et al. Pravastatin attenuates sepsis-induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of Cav-1/eNOS pathway. Int Immunopharmacol. 2021 Nov;100:108077Read more>>
  • Zhongxiao Zhou. et al. Formononetin regulates endothelial nitric oxide synthase to protect vascular endothelium in deep vein thrombosis rats:. INT J IMMUNOPATH PH. 2022;()Read more>>
  • Zhongxiao Zhou. et al. Formononetin regulates endothelial nitric oxide synthase to protect vascular endothelium in deep vein thrombosis rats:. INT J IMMUNOPATH PH. 2022;()Read more>>
  • Yang Wang. et al. Role of GADD45A in myocardial ischemia/reperfusion through mediation of the JNK/p38 MAPK and STAT3/VEGF pathways. INT J MOL MED. 2022 Dec;50(6):1-11Read more>>
  • Zhou Yanyan. et al. Homoplantaginin attenuates high glucose-induced vascular endothelial dysfunction via inhibiting store-operated calcium entry channel and endoplasmic reticulum stress. J PHARM PHARMACOL. 2023 SepRead more>>
VALIDATION IMAGES