bs-3597R-Cy3 [Conjugated Primary Antibody]
DDX4 Polyclonal Antibody, Cy3 Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: DDX4

Immunogen Range: 601-700/724


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 54514

Swiss Prot: Q9NQI0

Source: KLH conjugated synthetic peptide derived from human DDX4

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 80


Cross Reactive Species: Chicken

Predicted Cross Reactive Species: Human
Mouse
Rat
Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Imus, Nastassja, et al. "Transfer and Detection of Freshly Isolated or Cultured Chicken (Gallus gallus) and Exotic Species Embryonic Gonadal Germ Stem Cells in Host Embryos." Zoological Science 31.6 (2014): 360-368.Read more>>
  • Tang X et al. Retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) restore the germline competence of in vitro cultured chicken blastodermal cells. In Vitro Cell Dev Biol Anim. 2019 Mar;55(3):169-176.Read more>>
  • Qin X et al. Efficient knock-in at the chicken ovalbumin locus using adenovirus as a CRISPR/Cas9 delivery system. 3 Biotech,2019, 9(12). Read more>>
  • Nakami Wilkister Nabulindo. et al. Culture of Kenyan Goat (Capra hircus) Undifferentiated Spermatogonia in Feeder-Free Conditions. FRONT VET SCI. 2022; 9: 894075Read more>>
  • Xia, Yuguo. et al. Gut microbiota involved in spermatogenic function of Sancai Lianmei granules in obese mice. N-S ARCH PHARMACOL. 2022 Sep;:1-15Read more>>
  • Klein, Sabine. et al. Identification of chicken LOC420478 as Bucky ball equivalent and potential germ plasm organizer in birds. SCI REP-UK. 2022 Oct;12(1):1-15Read more>>
  • Yoshiharu Morimoto. et al. Mitochondrial Transfer into Human Oocytes Improved Embryo Quality and Clinical Outcomes in Recurrent Pregnancy Failure Cases. INT J MOL SCI. 2023 Jan;24(3):2738Read more>>
  • Wenqing Xu. et al. Oral exposure to polystyrene nanoplastics reduced male fertility and even caused male infertility by inducing testicular and sperm toxicities in mice. J HAZARD MATER. 2023 Jul;454:13147Read more>>