bs-3598R [Primary Antibody]
CXCR5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CXCR5/CD185

Immunogen Range: 281-372/372


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 643

Swiss Prot: P32302

Source: KLH conjugated synthetic peptide derived from human CXCR5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)

Predicted Molecular Weight: 45


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Huang, Hu, et al. "Age-related macular degeneration phenotypes are associated with increased tumor necrosis-alpha and subretinal immune cells in aged Cxcr5 knockout mice." PloS one 12.3 (2017): e0173716.Read more>>
  • Gong, Qiaoqiao, et al. "Increased levels of CCR7 (lo) PD‑1 (hi) CXCR5+ CD4+ T cells, and associated factors Bcl‑6, CXCR5, IL‑21 and IL‑6 contribute to repeated implantation failure." Experimental and Therapeutic Medicine 14.6 (2017): 5931-5941.Read more>>
  • Gong et al. Increased levels of CCR7(lo)PD-1(hi) CXCR5+CD4+T cells, and associated factors Bcl-6, CXCR5, IL-21 and IL-6 contribute to repeated implantation failure. (2017) Exp.Ther.Med. 14:5931-5941Read more>>
VALIDATION IMAGES

Lane 1: Mouse lymph node lysates probed with CXCR5 Polyclonal Antibody, Unconjugated (bs-3598R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.


Lane 1: Raji cell lysates; Lane 2: Mouse Lymph node lysates probed with CXCR5 Polyclonal Antibody, Unconjugated (bs-3598R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.