bs-3605R-Biotin [Conjugated Primary Antibody]
YAP1 Polyclonal Antibody, Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: YAP1

Immunogen Range: 131-330/504


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10413

Swiss Prot: P46937

Source: KLH conjugated synthetic peptide derived from human YAP1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 55


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Hongxin, et al. "gga-miR-375 Plays a Key Role in Tumorigenesis Post Subgroup J Avian Leukosis Virus Infection." PLoS One 9.4 (2014): e90878.Read more>>
  • Gao R et al. PM2.5-associated nitro-PAH exposure promotes tumor cell metastasis through Hippo-YAPmediated transcriptional regulation. Sci Total Environ. 2019 Aug 15;678:611-617. Read more>>
  • Sisi Liuet al. Geometric Confinement Guides the Expression of Cancer Stem Cell Molecular Markers CD44 via Cell Traction Forces. ACS Biomater Sci Eng. 2020 Aug 10;6(8):4623-4630.Read more>>
  • Liu J et al. Obesity Potentiates Esophageal Squamous Cell Carcinoma Growth and Invasion by AMPK-YAP PathwayJ Immunol Res.2020 Dec 10;2020:6765474.Read more>>
  • Qi Yang. et al. A novel biodegradable external stent regulates vein graft remodeling via the Hippo-YAP and mTOR signaling pathways. Biomaterials. 2020 Nov;258:120254Read more>>
  • Xinhao Wang. et al. Yes-associated protein reacts differently in vascular smooth muscle cells under different intensities of mechanical stretch. Aging-Us. 2022 Jan 15; 14(1): 286296Read more>>
  • Zixin Liu. et al. Polyisocyanide hydrogels with tunable nonlinear elasticity mediate liver carcinoma cell functional response. ACTA BIOMATER. 2022 JuRead more>>
  • Ruixing Liu. et al. Celastrol Alleviates Corneal Stromal Fibrosis by Inhibiting TGF-1/Smad2/3-YAP/TAZ Signaling After Descemet Stripping Endothelial Keratoplasty. INVEST OPHTH VIS SCI. 2023 Mar;64(3):9-9Read more>>
  • Ximin Yuan. et al. Tough Gelatin Hydrogel for Tissue Engineering. Advanced Science. 2023 Jun;:2301665Read more>>
  • Shao Zifei. et al. Exosomes derived from adipose tissues accelerate fibroblasts and keratinocytes proliferation and cutaneous wound healing via miR-92a/Hippo-YAP axis. J PHYSIOL BIOCHEM. 2023 Dec;:1-16Read more>>
  • Shun Zhang. et al. Hnrnpk Protects Against Osteoarthritis through Targeting WWC1 mRNA and Inhibiting Hippo Signaling Pathway. MOL THER. 2024 Feb 26Read more>>
  • Yiwei Wang. et al. M2 macrophages promote subconjunctival fibrosis through YAP/TAZ signalling. ANN MED. 2024 Feb 09Read more>>
VALIDATION IMAGES