Host: Rabbit
Target Protein: HSD11B2
Immunogen Range: 151-250/405
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3291
Swiss Prot: P80365
Source: KLH conjugated synthetic peptide derived from human HSD11B2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 45
Cross Reactive Species:
Human
Mouse
Rat
Horse
Predicted Cross Reactive Species: Dog
For research use only. Not intended for diagnostic or therapeutic use.
- Ding, Ying-xue, et al. "Regulation of glucocorticoid-related genes and receptors/regulatory enzyme expression in intrauterine growth restriction filial rats." Life Sciences (2016).Read more>>
- Xu et al. Preeclampsia is associated with a deficiency of lipoxin A4, an endogenous anti-inflammatory mediator. (2014) Fertil.Steri. 102:282-290.e4Read more>>
- Wang et al. Preeclampsia induced by cadmium in rats is related to abnormal local glucocorticoid synthesis in placenta. (2014) Reprod.Biol.Endocrino. 12:77Read more>>
- Haojing Liuet al. Glucocorticoid Exposure Induces Preeclampsia via DampeningLipoxin A4, an Endogenous Anti-Inflammatory and Proresolving Mediator. Front Pharmacol. 2020 Jul 28;11:1131.Read more>>
- Nakanishi Tomoya. et al. Cortisol induces follicle regression, while FSH prevents cortisol-induced follicle regression in pigs. Mol Hum Reprod. 2021 MayRead more>>
- Xue-Ting Shi. et al. Gestational cadmium exposure impairs placental angiogenesis via activating GC/GR signaling. Ecotox Environ Safe. 2021 Nov;224:112632Read more>>
Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded horse kidney labeled with Anti- HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney labeled with Anti- HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney labeled with Anti- HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Stomach tissue lysates; Lane 2: Rat Stomach tissue lysates probed with HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSD11B2) polyclonal Antibody, Unconjugated (bs-3618R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD11B2) Polyclonal Antibody, Unconjugated (bs-3618R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD11B2) Polyclonal Antibody, Unconjugated (bs-3618R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Stomach tissue lysates probed with FAP Polyclonal Antibody, Unconjugated (bs-3618R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.