bs-3619R-Cy3 [Conjugated Primary Antibody]
Talin Polyclonal Antibody, Cy3 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Talin1 + 2

Immunogen Range: 1601-1750/2541


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7094, 83660

Swiss Prot: Q9Y4G6, Q9Y490

Source: KLH conjugated synthetic peptide derived from human Talin 1.

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 280


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Dingyu, Wang, et al. "Regulation of Intracellular Structural Tension by Talin in the Axon Growth and Regeneration." Molecular Neurobiology (2015): 1-14.Read more>>
  • He, Jing, et al. "The essential role of inorganic substrate in the migration and osteoblastic differentiation of mesenchymal stem cells." Journal of the Mechanical Behavior of Biomedical Materials (2016).Read more>>
  • Yifan Wang. et al. Positive effect of Astragaloside IV on neurite outgrowth via talin\dependent integrin signaling and microfilament force. J Cell Physiol. 2021 Mar;236(3):2156-2168Read more>>
  • Xian Tang. et al. Expression of Talin-1 in endometriosis and its possible role in pathogenesis. Reprod Biol Endocrin. 2021 Dec;19(1):1-1Read more>>
VALIDATION IMAGES