bs-3662R [Primary Antibody]
WNT10B Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: WNT10B

Immunogen Range: 201-300/389


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7480

Swiss Prot: O00744

Source: KLH conjugated synthetic peptide derived from human WNT10b

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 40


Cross Reactive Species: Human
Mouse
Pig

Predicted Cross Reactive Species: Rat
Dog
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lu, Juan, et al. "Expression of Wnt3a, Wnt10b, β-catenin and DKK1 in periodontium during orthodontic tooth movement in rats." Acta Odontologica Scandinavica (2015): 1-7.Read more>>
  • Zhang, H., et al. "Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling."Cellular Physiology and Biochemistry 39.1 (2016): 360-370.Read more>>
  • Ke, Jin, et al. "Erbium: YAG laser (2,940 nm) treatment stimulates hair growth through upregulating Wnt 10b and β-catenin expression in C57BL/6 mice." International Journal of Clinical and Experimental Medicine 8.11 (2015): 20883.Read more>>
  • Zhang et al. Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling. (2016) Cell.Physiol.Biochem. 39:360-70Read more>>
  • Li M et al. Plant MIR156 Regulates Intestinal Growth in Mammals by Targeting the Wnt/β-catenin Pathway. Am J Physiol Cell Physiol. 2019 Jun 5. Read more>>
  • Liya Bai. et al. DNA methylation and histone acetylation are involved in Wnt10b expression during the secondary hair follicle cycle in Angora rabbits. 2021 Jan 06Read more>>
  • Subin Jeong. et al. Optimization of hair follicle spheroids for the hair-on-a-chip. BIOMATER SCI-UK. 2024 FebRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT10B) Polyclonal Antibody, Unconjugated (bs-3662R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.