bs-3674R [Primary Antibody]
PBR Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PBR

Immunogen Range: 31-130/169


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1622

Source: KLH conjugated synthetic peptide derived from human Peripheral type Benzodiazepine Receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

PBR (Peripheral type Benzodiazepine Receptor) is a ubiquitous protein (18 kDa) found in most steroidogenic tissues. It is located primarily in the outer mitochondrial membrane of steroid producing tissues and is found in association with a 34 kDa voltage dependent anion channel protein (VDAC). PBR interacts with some benzodiazepines and has different affinities than its endogenous counterpart. PBR appears to be a key factor in the transport of cholesterol from the outer to the inner mitochondrial membrane to permit the initiation of steroid hormone synthesis. It is thought to be part of the mitochondrial permeability transition pore (PT) and to be involved in apoptosis. It is speculated that patients with congenital lipoid adrenal hyperplasia, who cannot make any steroids, might have a genetic lesion in BZRP. A short form, PBRS is also expressed in the same tissues, but at a level about ten times that of PBR.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 18


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wenqian Xie. et al. Seasonal expressions of the translocator protein (18?kDa), voltage-dependent anion channel, and steroidogenic acute regulatory protein in the scent glands of muskrats (Ondatra zibethicus). J STEROID BIOCHEM. 2023 Nov;234:1064Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PBR Polyclonal Antibody, Unconjugated (bs-3674R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PBR Polyclonal Antibody, Unconjugated (bs-3674R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PBR Polyclonal Antibody, Unconjugated (bs-3674R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TSPO/PBR) Polyclonal Antibody, Unconjugated (bs-3674R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.