bs-3737R-A647 [Conjugated Primary Antibody]
PPAR Gamma (ser112) Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PPAR Gamma ser112

Modification Site: ser112

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5468

Swiss Prot: P37231

Source: KLH conjugated synthetic phosphopeptide derived from human PPAR Gamma around the phosphorylation site of ser112

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses.

Conjugation: ALEXA FLUOR® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 57


Cross Reactive Species: Human
Mouse
Rat
Dog

Predicted Cross Reactive Species: Pig
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mölzer, Christine, et al. "Features of an altered AMPK metabolic pathway in Gilbert’s Syndrome, and its role in metabolic health." Scientific Reports 6 (2016): 30051.Read more>>
  • Manabe A et al.Upregulation of transient receptor potential melastatin 6 channel expression by rosiglitazone and all-trans-retinoic acid in erlotinib-treated renal tubular epithelial cells.(2018)J Cell Physiol. Read more>>
  • Liu Y et al. Telmisartan inhibits oxalate and calcium oxalate crystal-induced epithelial-mesenchymal transformation via PPAR-γ-AKT/STAT3/p38 MAPK-Snail pathway. Life Sci. 2019 Nov 28;241:117108.Read more>>
  • Min Zhao et al. HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway. Mediators of Inflammation, 2019, 1–17. Read more>>
  • Liu YD et al. Rosiglitazone Suppresses Calcium Oxalate Crystal Binding and Oxalate-Induced Oxidative Stress in Renal Epithelial Cells by Promoting PPAR-γ Activation and Subsequent Regulation of TGF-β1 and HGF Expression. Oxid Med Cell Longev. 2019 Nov 12;2019:4826525.Read more>>
  • Hsu MH et al. Effects of Maternal Resveratrol on Maternal High-Fat Diet/Obesity with or without Postnatal High-Fat Diet. Int J Mol Sci. 2020 May 12;21(10):3428.Read more>>
  • Yurong Zhao. et al. Deoxynivalenol Exposure Suppresses Adipogenesis by Inhibiting the Expression of Peroxisome Proliferator-Activated Receptor Gamma 2 (PPAR2) in 3T3-L1 Cells. Int J Mol Sci. 2020 Jan;21(17):63Read more>>
  • Fadian Ding. et al. Adropin attenuates pancreatitis?associated lung injury through PPAR phosphorylation?related macrophage polarization. INT J MOL MED. 2023 Oct;52(4):1-1Read more>>
VALIDATION IMAGES