bs-3741R [Primary Antibody]
HSF1(Ser326) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HSF1 Ser326

Modification Site: Ser326

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3297

Swiss Prot: Q00613

Source: KLH conjugated synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of Ser326

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 57


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Evert, M., et al. "Deregulation of DNA-dependent protein kinase catalytic subunit contributes to human hepatocarcinogenesis development and has a putative prognostic value." British Journal of Cancer (2013).Read more>>
  • Frinchi,et al.Heat shock protein (Hsp) regulation by muscarinic acetylcholine receptor (mAChR) activation in the rat hippocampus.(2018) Journal of Cellular Physiology. 233:6107-6116.Read more>>
  • Kodet,et al.Microenvironment‑driven resistance to B‑Raf inhibition in a melanoma patient is accompanied by broad changes of gene methylation and expression in distal fibroblasts.(2018) International Journal of Molecular Medicine. 41:2687-2703.Read more>>
  • Weikun Qian. et al. The EGFR-HSF1 axis accelerates the tumorigenesis of pancreatic cancer. J Exp Clin Canc Res. 2021 Dec;40(1):1-19Read more>>
  • Tamara Isermann. et al. Suppression of HSF1 activity by wildtype p53 creates a driving force for p53 loss-of-heterozygosity. Nat Commun. 2021 Jun;12(1):1-17Read more>>
  • Hoora Shaghaghi. et al. A model of the aged lung epithelium in idiopathic pulmonary fibrosis. Aging-Us. 2021 Jul 15; 13(13): 16922C16937Read more>>
  • Miriana Scordino. et al. Manipulation of HSP70-SOD1 Expression Modulates SH-SY5Y Differentiation and Susceptibility to Oxidative Stress-Dependent Cell Damage: Involvement in Oxotremorine-M-Mediated Neuroprotective Effects. ANTIOXIDANTS-BASEL. 2023 Mar;12(3):687Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti phospho-HSF1(Ser326)Polyclonal Antibody, Unconjugated (bs-3741R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-HSF1(Ser326)Polyclonal Antibody, Unconjugated (bs-3741R) at 1:200 followed by conjugation to the secondary antibody and DAB staining