bs-3744R-A750

DATASHEET

Host: Rabbit

Target Protein: Paxillin Tyr31

Specificity: A mismatch around the phosphorylation site decreases the antibody's affinity in Mouse/Rat samples

Modification Site: Tyr31

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5829

Swiss Prot: P49023

Source: KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr31

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).

Conjugation: ALEXA FLUOR® 750

Excitation/ Emission: 749nm/775nm

Size: 100ul

Concentration: 1ug/ul

Applications: IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 64

Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Sheep
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.