bs-3790R [Primary Antibody]
GM-CSF Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GM-CSF

Immunogen Range: 65-144/144

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1437

Swiss Prot: P04141

Source: KLH conjugated synthetic peptide derived from human GM-CSF

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage Condition: Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()

Predicted Molecular Weight: 14

Predicted Cross Reactive Species: Human
Mouse
Rat
Dog
Cow
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Zhen, et al. "Methods for transferring gene into silkworm and expression of hGM-CSF in silk glands of transgenic silkworm." Journal of Chemical and Pharmaceutical Research 6.5 (2014): 118-129.Read more>>
  • Anzai, Atsushi, et al. "The infarcted myocardium solicits GM-CSF for the detrimental oversupply of inflammatory leukocytes." Journal of Experimental Medicine (2017): jem-20170689.Read more>>
  • Hilgendorf et al. Innate response activator B cells aggravate atherosclerosis by stimulating T helper-1 adaptive immunity. (2014) Circulatio. 129:1677-87Read more>>
  • Anzai et al. The infarcted myocardium solicits GM-CSF for the detrimental oversupply of inflammatory leukocytes. (2017) J.Exp.Med. 214:3293-3310Read more>>
  • Papp KA et al. GM-CSF as a therapeutic target in psoriasis: randomised, controlled investigation using namilumab - a specific, human anti-GM-CSF monoclonal antibody.(2018) Br. J. Dermatol. Sep 12Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti GM-CSF Polyclonal Antibody, Unconjugated (bs-3790R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Recombined Trx/His-tag porcine GM-CSF protein; Lane 2: Recombined His-tag porcine GM-CSF protein probed with GM-CSF Polyclonal Antibody, Unconjugated (bs-3790R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.