bs-3793R [Primary Antibody]
CD75 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD75

Immunogen Range: 301-406/406


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6480

Swiss Prot: P15907

Source: KLH conjugated synthetic peptide derived from human CD75

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi apparatus but which can be proteolytically processed to a soluble form, is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human spleen labeled with Anti CD75 Polyclonal Antibody, Unconjugated (bs-3793R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti CD75 Polyclonal Antibody, Unconjugated (bs-3793R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: MCF-7 cell lysates;Lane 2: A549 cell lysates;Lane 3: A431 cell lysates; Lane 4: Siha cell lysates probed with CD75 Polyclonal Antibody, Unconjugated (bs-3793R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.