bs-3816R [Primary Antibody]
Oct-4B(OCT4B-190)Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Oct-4BOCT4B-190NT

Immunogen Range: 201-300/360

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5460

Swiss Prot: Q01860

Source: KLH conjugated synthetic peptide derived from human OCT4B-190

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Yu-Hong, et al. "A signature for induced pluripotent stem cell?Cassociated genes in colorectal cancer."Medical Oncology 30.1 (2013): 1-11.eRead more>>
  • Darabi, Shahram, et al. "Polarized neural stem cells derived from adult bone marrow stromal cells develop a rosette-like structure." In Vitro Cellular & Developmental Biology-Animal (2013): 1-15.Read more>>
  • Tanabe, A., et al. "Radioresistance of cancer stem‐like cell derived from canine tumours." Veterinary and Comparative Oncology (2014).Read more>>
  • Liu, Lu, et al. "OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation." BioMed Research International 2017 (2017).Read more>>
  • Liu et al. JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells. (2015) Acta.Pharmacol.Si. 36:1099-106Read more>>
  • Liu et al. OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation. (2017) Biomed.Res.Int. 2017:2756891Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colorectal cancer labeled with Anti OCT4B-190 Polyclonal Antibody, Unconjugated (bs-3816R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti OCT4B-190 Polyclonal Antibody, Unconjugated (bs-3816R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


RSC96 probed with Oct-4B(OCT4B-190)Polyclonal Antibody, Unconjugated (bs-3816R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Cerebellum lysates; Lane 4: Mouse Testis lysates; Lane 5: Rat Cerebrum lysates; Lane 6: Rat Heart lysates; Lane 7: Rat Cerebellum lysates probed with Oct-4B(OCT4B-190)Polyclonal Antibody, Unconjugated (bs-3816R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Oct-4B(OCT4B-190) Polyclonal Antibody, Unconjugated (bs-3816R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Oct-4B(OCT4B-190) Polyclonal Antibody, Unconjugated (bs-3816R) at 1:200 overnight at 4°C, DAB staining.