bs-3828R [Primary Antibody]
HNF4A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HNF4A

Immunogen Range: 301-400/464


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3172

Swiss Prot: P41235

Source: KLH conjugated synthetic peptide derived from human HNF4 alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang C et al. Caveolin-1 promotes Rfng expression via Erk-Jnk-p38 signaling pathway in mouse hepatocarcinoma cells. J Physiol Biochem. 2019 Sep 16.Read more>>
  • Johanna Vollmar. et al. Functional inhibition of Oct leads to HNF4 upregulation. Exp Ther Med. 2021 Apr;21(4):1-1Read more>>
  • Lv, Xiaoqin. et al. GATA binding protein 4 promotes the expression and transcription of hepatitis B virus by facilitating hepatocyte nuclear factor 4 alpha in vitro. Virol J. 2021 Dec;18(1):1-12Read more>>
  • Qidang Duan. et al. LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4 signaling in HLSECs. HELIYON. 2022 Dec;8:e12385Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney labeled with Anti-HNF4/TCF4/HNF4A Polyclonal Antibody, Unconjugated (bs-3828R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


BRL-3A cell lysates probed with HNF4A Polyclonal Antibody, Unconjugated (bs-3828R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hcclm3 cell lysates probed with HNF4A Polyclonal Antibody, Unconjugated (bs-3828R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.