bs-3847R [Primary Antibody]
Amphiregulin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Amphiregulin

Immunogen Range: 185-252/252


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 374

Swiss Prot: P15514

Source: KLH conjugated synthetic peptide derived from human Amphiregulin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
FCM(1:100)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100)

Predicted Molecular Weight: 16


Cross Reactive Species: Human
Mouse
Rat
Guinea Pig

Predicted Cross Reactive Species: Dog
Cow
Sheep
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jiang, Wen Jun, et al. "Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia." EBioMedicine (2017).Read more>>
  • c "Evaluation of estrogenic potency of a standardized hops extract on mammary gland biology and on MNU-induced mammary tumor growth in rats." The Journal of Steroid Biochemistry and Molecular Biology (2017).Read more>>
  • Liu et al. Mast Cells Participate in Corneal Development in Mice. (2015) Sci.Re. 5:17569Read more>>
  • Oka M et al. Dedifferentiation of smooth muscle cells in intracranial aneurysms and its potential contribution to the pathogenesis. Sci Rep. 2020 May 20;10(1):8330.Read more>>
  • Seung Seob Son. et al. In vivo silencing of amphiregulin by a novel effective Self-Assembled-Micelle inhibitory RNA ameliorates renal fibrosis via inhibition of EGFR signals. Sci Rep-Uk. 2021 Jan;11(1):1-14Read more>>
  • Mukharram M. Bikbov. et al. Compatibility of intravitreally applied epidermal growth factor and amphiregulin. 2021 Mar 13Read more>>
  • onnie Douglas. et al. Transgenic expression of a T cell epitope in Strongyloides ratti reveals that helminth-specific CD4+ T cells constitute both Th2 and Treg populations. Plos Pathog. 2021 Jul;17(7):e1009709Read more>>
  • Huihua Kai. et al. LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6. Front Cell Dev Biol. 2021; 9: 701628Read more>>
  • Man She. et al. Dynamic Changes of AREG in the Sclera during the Development of Form-Deprivation Myopia in Guinea Pigs. 2021 Nov 12Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon cancer labeled with Anti Amphiregulin Polyclonal Antibody, Unconjugated (bs-3847R) at 1:600 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded mouse fetal liver labeled with Rabbit Anti-Amphiregulin Polyclonal Antibody, Unconjugated(bs-3847R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.\n


Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.