Host: Rabbit
Target Protein: Amphiregulin
Immunogen Range: 185-252/252
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 374
Swiss Prot: P15514
Source: KLH conjugated synthetic peptide derived from human Amphiregulin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 16
Cross Reactive Species:
Human
Mouse
Rat
Guinea Pig
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Jiang, Wen Jun, et al. "Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia." EBioMedicine (2017).Read more>>
- c "Evaluation of estrogenic potency of a standardized hops extract on mammary gland biology and on MNU-induced mammary tumor growth in rats." The Journal of Steroid Biochemistry and Molecular Biology (2017).Read more>>
- Liu et al. Mast Cells Participate in Corneal Development in Mice. (2015) Sci.Re. 5:17569Read more>>
- Oka M et al. Dedifferentiation of smooth muscle cells in intracranial aneurysms and its potential contribution to the pathogenesis. Sci Rep. 2020 May 20;10(1):8330.Read more>>
- Seung Seob Son. et al. In vivo silencing of amphiregulin by a novel effective Self-Assembled-Micelle inhibitory RNA ameliorates renal fibrosis via inhibition of EGFR signals. Sci Rep-Uk. 2021 Jan;11(1):1-14Read more>>
- Mukharram M. Bikbov. et al. Compatibility of intravitreally applied epidermal growth factor and amphiregulin. 2021 Mar 13Read more>>
- onnie Douglas. et al. Transgenic expression of a T cell epitope in Strongyloides ratti reveals that helminth-specific CD4+ T cells constitute both Th2 and Treg populations. Plos Pathog. 2021 Jul;17(7):e1009709Read more>>
- Huihua Kai. et al. LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6. Front Cell Dev Biol. 2021; 9: 701628Read more>>
- Man She. et al. Dynamic Changes of AREG in the Sclera during the Development of Form-Deprivation Myopia in Guinea Pigs. 2021 Nov 12Read more>>
- Man She. et al. AREG is involved in scleral remodeling in form-deprivation myopia via the ERK1/2-MMP-2 pathway. FASEB J. 2022 May;36(5):e22289Read more>>
Formalin-fixed and paraffin embedded human colon cancer labeled with Anti Amphiregulin Polyclonal Antibody, Unconjugated (bs-3847R) at 1:600 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded mouse fetal liver labeled with Rabbit Anti-Amphiregulin Polyclonal Antibody, Unconjugated(bs-3847R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.\n
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Breast tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Rat Placenta tissue lysates; Lane 5: Rat Breast tissue lysates; Lane 6: Rat Testis tissue lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human HepG2 cell lysates; Lane 9: Human A431 cell lysates probed with Amphiregulin Polyclonal Antibody, Unconjugated (bs-3847R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.