bs-3884R [Primary Antibody]
GPX4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GPX4

Immunogen Range: 101-197/197


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2879

Source: KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 22


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sukfan P. Kwong. et al. PORIMIN: The key to (+)-Usnic acid-induced liver toxicity and oncotic cell death in normal human L02 liver cells. J Ethnopharmacol. 2021 Apr;270:113873Read more>>
  • Jing Ge. et al. Comparative study on protective effect of different selenium sources against cadmium-induced nephrotoxicity via regulating the transcriptions of selenoproteome. Ecotox Environ Safe. 2021 Jun;215:112135Read more>>
  • Andrea Krehe?ov. et al. Characterization of Glutathione Peroxidase 4 in Rat Oocytes, Preimplantation Embryos, and Selected Maternal Tissues during Early Development and Implantation. Int J Mol Sci. 2021 Jan;22(10):5174Read more>>
  • Xiao Han. et al. ZEB1 directly inhibits GPX4 transcription contributing to ROS accumulation in breast cancer cells. Breast Cancer Res Tr. 2021 Jul;188(2):329-342Read more>>
  • Tao Liu. et al. Multifaceted roles of a bioengineered nanoreactor in repressing radiation-induced lung injury. Biomaterials. 2021 Oct;277:121103Read more>>
  • Lijuan Bao. et al. Ferritinophagy is involved in Bisphenol A-induced ferroptosis of renal tubular epithelial cells through the activation of the AMPK-mTOR-ULK1 pathway. Food Chem Toxicol. 2022 May;163:112909Read more>>
  • Jianwei Lei. et al. The protective mechanism of protein kinase R to inhibit neuronal ferroptosis in cerebral injury from subarachnoid hemorrhage. BRAIN BEHAV. 2022 Jul;:e2722Read more>>
  • Huang Bin. et al. Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 Stabilization. OXID MED CELL LONGEV. 2022;2022:9735555Read more>>
  • Zhaoqi He. et al. Cadmium induces liver dysfunction and ferroptosis through the endoplasmic stress-ferritinophagy axis. ECOTOX ENVIRON SAFE. 2022 Oct;245:114123Read more>>
  • Zhang Kaixin. et al. MiR-129-3p regulates ferroptosis in the liver of Selenium-deficient broilers by targeting SLC7A11. POULTRY SCIENCE. 2022 Oct;:102271Read more>>
  • Hu, Xiaomeng. et al. PNO1 inhibits autophagy-mediated ferroptosis by GSH metabolic reprogramming in hepatocellular carcinoma. CELL DEATH DIS. 2022 Nov;13(11):1-12Read more>>
  • Yajing Li. et al. Effect of Zearalenone-Induced Ferroptosis on Mice Spermatogenesis. ANIMALS. 2022 Jan;12(21):3026Read more>>
  • Shuang Wang. et al. Dioscin Protects against Cisplatin-Induced Acute Kidney Injury by Reducing Ferroptosis and Apoptosis through Activating Nrf2/HO-1 Signaling. ANTIOXIDANTS-BASEL. 2022 Dec;11(12):2443Read more>>
  • Ke Li. et al. Oxygen Self-Generating Nanoreactor Mediated Ferroptosis Activation and Immunotherapy in Triple-Negative Breast Cancer. ACS NANO. 2023;17(5):4667C4687Read more>>
  • Xu Chen. et al. Chiral Ruthenium Nanozymes with Self-Cascade Reaction Driven the NO Generation Induced Macrophage M1 Polarization Realizing the Lung Cancer Cocktail Therapy. SMALL. 2023 Apr;:2207823Read more>>
  • Lu Yu. et al. Chronic arsenic exposure induces ferroptosis via enhancing ferritinophagy in chicken livers. SCI TOTAL ENVIRON. 2023 May;:164172Read more>>
  • Li Zhang. et al. Tumor-Associated Macrophages Regulating a Polymer Nanoplatform for Synergistic Treatment of Breast Tumors. ACS APPL MATER INTER. 2023;15(29):34527C34539Read more>>
  • Yu-Sheng Shi. et al. Dendrobine rescues cognitive dysfunction in diabetic encephalopathy by inhibiting ferroptosis via activating Nrf2/GPX4 axis. PHYTOMEDICINE. 2023 Jul;:154993Read more>>
  • Zhong Gaolong. et al. Copper Exposure Induced Chicken Hepatotoxicity: Involvement of Ferroptosis Mediated by Lipid Peroxidation, Ferritinophagy, and Inhibition of FSP1-CoQ10 and Nrf2/SLC7A11/GPX4 Axis. BIOL TRACE ELEM RES. 2023 Jul;:1-11Read more>>
  • Mali Qiu. et al. YAP facilitates NEDD4L-mediated ubiquitination and degradation of ACSL4 to alleviate ferroptosis in myocardial ischemia-reperfusion injury. CAN J CARDIOL. 2023 AugRead more>>
  • Yang Liu. et al. Tuberostemonine may enhance the function of the SLC7A11/glutamate antiporter to restrain the ferroptosis to alleviate pulmonary fibrosis. J ETHNOPHARMACOL. 2023 Aug;:116983Read more>>
  • Yueqi Yang. et al. A Compared Study of Eicosapentaenoic Acid and Docosahexaenoic Acid in Improving Seizure-Induced Cognitive Deficiency in a Pentylenetetrazol-Kindling Young Mice Model. MAR DRUGS. 2023 Sep;21(9):464Read more>>
  • Lihua Zhao. et al. Saikosaponin A alleviates Staphylococcus aureus-induced mastitis in mice by inhibiting ferroptosis via SIRT1/Nrf2 pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 AugRead more>>
  • Ting Hong. et al. Exosomal circBBS2 inhibits ferroptosis by targeting miR-494 to activate SLC7A11 signaling in ischemic stroke. FASEB J. 2023 Aug;37(9):e23152Read more>>
  • Huan Yang. et al. Heat stress induces ferroptosis of porcine Sertoli cells by enhancing CYP2C9-Ras- JNK axis. THERIOGENOLOGY. 2024 Feb;215:281Read more>>
  • Yang Liu. et al. Inhibition of Oxidative Stress-Induced Ferroptosis Can Alleviate Rheumatoid Arthritis in Human. J IMMUNOL RES. 2024;2024:9943747Read more>>
VALIDATION IMAGES

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Heart tissue lysates ; Lane 5: Rat Testis tissue lysates ; Lane 6: Rat Liver tissue lysates; Lane 7: Rat Kidney tissue lysates; Lane 8: Rat Heart tissue lysates; Lane 9: U937 cell lysates; Lane 10: HepG2 cell lysates; Lane 11: MCF-7 cell lysates; Lane 12: Huvec cell lysates; Lane 13: 293T cell lysates probed with GPX4 Polyclonal Antibody, Unconjugated (bs-3884R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.