Host: Rabbit
Target Protein: NCF1/p47 phox
Immunogen Range: 151-250/390
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 653361
Swiss Prot: P14598
Source: KLH conjugated synthetic peptide derived from human NCF1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 45
Cross Reactive Species:
Human
Mouse
Horse
Predicted Cross Reactive Species:
Rat
Dog
For research use only. Not intended for diagnostic or therapeutic use.
- Wang et al. Identification of a specific α-synuclein peptide (α-Syn 29-40) capable of eliciting microglial superoxide production to damage dopaminergic neurons. (2016) J.Neuroinflammation. 13:158Read more>>
- Mukohda M et al. Increased Blood Pressure Causes Lymphatic Endothelial Dysfunction via Oxidative Stress in Spontaneously Hypertensive Rats. Hypertension. 2020 Aug;76(2):598-606.Read more>>
- Chunming Xu. et al. Vitamin B ameliorates PM2.5-induced kidney damage by reducing endoplasmic reticulum stress and oxidative stress in pregnant mice and HK-2. TOXICOLOGY. 2023 May;:153568Read more>>
Lane 1: human colon carcinoma lysates Lane 2: mouse brain lysates probed with Anti NCF1/p47 phox Polyclonal Antibody, Unconjugated (bs-3886R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 45kD. Observed band size: 45kD
Mouse bone, lymph node, and spleen lysates probed with Polyclonal Antibody, unconjugated (bs-3886R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
U-937 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NCF1/p47 phox Polyclonal Antibody(bs-3886R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Lymph node lysates probed with NCF1/p47 phox Polyclonal Antibody, Unconjugated (bs-3886R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.