bs-3889R
[Primary Antibody]
NOX2/gp91phox Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: NOX2/gp91phox
Immunogen Range: 501-570/570
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1536
Swiss Prot: P04839
Source: KLH conjugated synthetic peptide derived from human NOX2/gp91phox
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 65
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Yabin, et al. "Activation of Liver X receptor improves viability of adipose-derived mesenchymal stem cells (AD-MSCs) to attenuate myocardial ischemia injury through TLR4/NF-κB and Keap-1/Nrf2 signaling pathways." Antioxidants & Redox Signaling ja (2014).Read more>>
- Kashiwabara, Yoshiaki, et al. "Immunohistochemical localization of Nox in mouse circumvallate papillae." Tissue and Cell (2015).Read more>>
- Wakatsuki, Shuji, et al. "Oxidative stress–dependent phosphorylation activates ZNRF1 to induce neuronal/axonal degeneration." The Journal of Cell Biology (2015): jcb-201506102.Read more>>
- Hu, Xiaoqin, et al. "Effects of Resveratrol on Oxidative Stress Injury Induced by Rapid-Pacing in Isolated Rabbit Hearts." Journal of Developing Drugs 3.128 (2014): 2.Read more>>
- Lagadinou, Eleni D., et al. "BCL-2 inhibition targets oxidative phosphorylation and selectively eradicates quiescent human leukemia stem cells." Cell stem cell 12.3 (2013): 329-341.Read more>>
- Ye, Zhen-Nan, et al. "Inhibition of leukotriene B4 synthesis protects against early brain injury possibly via reducing the neutrophil-generated inflammatory response and oxidative stress after subarachnoid hemorrhage in rats." Behavioural Brain Research (2017).Read more>>
- Köroğlu KM et al. Apocynin alleviates cisplatin‐induced testicular cytotoxicity by regulating oxidative stress and apoptosis in rats. Andrologia. 2019 May;51(4):e13227. Read more>>
- Chi Q et al. Hydrogen Sulfide Gas Exposure Induces Necroptosis and Promotes Inflammation through the MAPK/NF-κB Pathway in Broiler Spleen. Oxid Med Cell Longev. 2019 Jul 31;2019:8061823. Read more>>
- Liu HB et al. Celastrol alleviates aortic valve calcification via inhibition of NADPH oxidase-2 in valvular interstitial cells. JACC: Basic to Translational Science. 18 December 2019.Read more>>
- Yuan X et al. Aldosterone promotes renal interstitial fibrosis via the AIF‑1/AKT/mTOR signaling pathway. Mol Med Rep. 2019 Sep 13.Read more>>
- Zhao Y et al. Walnut Polyphenol Extract Protects against Malathion- and Chlorpyrifos-Induced Immunotoxicity by Modulating TLRx-NOX-ROS. Nutrients. 2020 Feb 27;12(3). pii: E616. Read more>>
- Xue-Qi Liuet al. Rutaecarpine Derivative Cpd-6c Alleviates Acute Kidney Injury by Targeting PDE4B, a Key Enzyme Mediating inflammation in Cisplatin Nephropathy. Biochem Pharmacol. 2020 Oct;180:114132.Read more>>
- Zixuan Liu. et al. Tetrachlorobenzoquinone exposure triggers ferroptosis contributing to its neurotoxicity. Chemosphere. 2021 Feb;264:128413Read more>>
- Cheng Yu. et al. Exposure to maternal diabetes induces endothelial dysfunction and hypertension in adult male rat offspring. Microvasc Res. 2021 Jan;133:104076Read more>>
- Yun Li. et al. The voltage-gated proton channel Hv1 plays a detrimental role in contusion spinal cord injury via extracellular acidosis-mediated neuroinflammation. Brain Behav Immun. 2021 Jan;91:267Read more>>
- Rodney M. Ritzel. et al. Proton extrusion during oxidative burst in microglia exacerbates pathological acidosis following traumatic brain injury. Glia. 2021 Mar;69(3):746-764Read more>>
- Zixuan Liu. et al. Iron-bearing nanoparticles trigger human umbilical vein endothelial cells ferroptotic responses by promoting intracellular iron level. Environ Pollut. 2021 Oct;287:117345Read more>>
- Xiaoyu Liu. et al. Geniposide Combined With Notoginsenoside R1 Attenuates Inflammation and Apoptosis in Atherosclerosis via the AMPK/mTOR/Nrf2 Signaling Pathway. Front Pharmacol. 2021; 12: 687394Read more>>
- Mr. Aleksandr Vendrov. et al. Renal NOXA1/NOX1 Signaling Regulates Epithelial Sodium Channel and Sodium Retention in Angiotensin II-induced Hypertension. 2021 Oct 29Read more>>
- Yun Li. et al. Sexual dimorphism in neurological function after SCI is associated with disrupted neuroinflammation in both injured spinal cord and brain. Brain Behav Immun. 2022 Mar;101:1Read more>>
- Lei Chen. et al. Therapeutic Potential of Perillaldehyde in Ameliorating Vulvovaginal Candidiasis by Reducing Vaginal Oxidative Stress and Apoptosis. Antioxidants-Basel. 2022 Feb;11(2):178Read more>>
- Rukang Zhang. et al. Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells. CELL CHEM BIOL. 2022 ApRead more>>
- Xin Zhao. et al. Diammonium glycyrrhizinate ameliorates portal hypertension by regulating portal macrophage oxidation and superoxide dismutase 3. EUR J PHARMACOL. 2022 Jun;:175115Read more>>
- Xu Zhou. et al. Magnesium isoglycyrrhizinate ameliorates lipopolysaccharide-induced liver injury by upregulating autophagy and inhibiting inflammation via IL-22 expression. BIOORG CHEM. 2022 Nov;128:106034Read more>>
- Pu Tian-Tian. et al. Evaluation of coenzyme Q10 (CoQ10) deficiency and therapy in mouse models of cardiomyopathy. J CARDIOVASC PHARM. 2023 Jan;:10.1097/FJC.0000000000001401Read more>>
- Rodney M. Ritzel. et al. Brain injury accelerates the onset of a reversible age-related microglial phenotype associated with inflammatory neurodegeneration. SCI ADV. 2023 Mar;9(10)Read more>>
- Germn Higuera-Martnez. et al. Apocynin, an NADPH Oxidase Enzyme Inhibitor, Prevents Amebic Liver Abscess in Hamster. BIOMEDICINES. 2023 Aug;11(8):2322Read more>>
- Minglu Xu. et al. The Nrf2 Pathway Alleviates Overloading Force-Induced TMJ Degeneration by Downregulating Oxidative Stress Reactions. J INFLAMM RES. 2023 Nov 26Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-NOX2/gp91phox Polyclonal Antibody, Unconjugated (bs-3889R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Formalin-fixed and paraffin embedded rat pancreas labeled with Anti NOX2/gp91phox Polyclonal Antibody, Unconjugated (bs-3889R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with NOX2/gp91phox Polyclonal Antibody, unconjugated (bs-3889R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Lane 1: Rat Bronchus tissue lysates; Lane 2: Rat Esophagus tissue lysates; Lane 3: Human HL60 cell lysates probed with NOX2 Polyclonal Antibody, Unconjugated (bs-3889R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.