Host: Rabbit
Target Protein: CYP11B1
Immunogen Range: 301-400/503
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1584
Swiss Prot: P15538
Source: KLH conjugated synthetic peptide derived from human CYP11B1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 55
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Tyczewska et al. Expression of selected genes involved in steroidogenesis in the course of enucleation-induced rat adrenal regeneration. (2014) Int.J.Mol.Me. 33:613-23Read more>>
- Nakanishi Tomoya. et al. Cortisol induces follicle regression, while FSH prevents cortisol-induced follicle regression in pigs. Mol Hum Reprod. 2021 MayRead more>>
- Vagnerov Karla. et al. Microbiota modulates the steroid response to acute immune stress in male mice. FRONT IMMUNOL. 2024 Feb;15Read more>>
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CYP11B1 Polyclonal Antibody, Unconjugated (bs-3898R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat thyroid gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CYP11B1 Polyclonal Antibody, Unconjugated (bs-3898R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
Mouse heart lysates probed with CYP11B1 Polyclonal Antibody, Unconjugated (bs-3898R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.