bs-3906R [Primary Antibody]
HSD3B1 Polyclonal Antibody
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Host: Rabbit

Target Protein: HSD3B1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3283

Source: KLH conjugated synthetic peptide derived from human HSD3B1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

HSD3B1 (3-beta hydroxysteroid dehydrogenase) is a bifunctional enzyme that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.There are two HSD3B1 proteins, type I and type II. Expressed by different genes, they function in different areas of the body. HSD3B1 has been shown to be a highly specific and sensitive trophoblast-associated marker.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Daojuan Wanget al. Exposure to hyperandrogen drives ovarian dysfunction and fibrosis by activating the NLRP3 inflammasome in mice. Sci Total Environ. 2020 Nov 25;745:141049.Read more>>
  • Xiyan Wang. et al. The optimized research of the in vitro culture of preantral follicles in mice. J Clin Lab Anal. 2020 Nov;34(11):e23498Read more>>
  • Wanhong Li. et al. Dietary supplementation of grape seed tannin extract stimulated testis development, changed fatty acid profiles and increased testis antioxidant capacity in pre-puberty hu lambs. Theriogenology. 2021 Sep;172:16Read more>>
  • Lijun Dai. et al. The Distribution, Expression Patterns and Functional Analysis of NR1D1 and NR4A2 in the Reproductive Axis Tissues of the Male Tianzhu White Yak. Animals-Basel. 2021 Nov;11(11):3117Read more>>
  • Lijun Dai. et al. The Distribution, Expression Patterns and Functional Analysis of NR1D1 and NR4A2 in the Reproductive Axis Tissues of the Male Tianzhu White Yak. Animals-Basel. 2021 Nov;11(11):3117Read more>>
  • Yang, Meixia. et al. Cytosine-phosphate-guanine oligodeoxynucleotides regulate the cell cycle, apoptosis, and steroidogenesis of mouse ovarian granulosa cells by targeting inhibin alpha (1_~_32) fragments. IN VITRO CELL DEV-AN. In Vitro Cell Dev-An. 2022 Apr;:1-12Read more>>
  • Zeqi Tang. et al. Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (Ondatra zibethicus). EUR J HISTOCHEM. 2022 Mar 24; 66(2): 3398Read more>>
  • Zihui Wang. et al. Heat stress and hypoxia inhibit the secretion of androgens and induce epithelial-to-mesenchymal transition associated with activated TGF-_/Smad signaling in canine cryptorchidism. REPROD DOMEST ANIM. 2022 Jun 09Read more>>
  • Hui Zhao. et al. Alleviating effects of selenium on fluoride-induced testosterone synthesis disorder and reproduction toxicity in rats. ECOTOX ENVIRON SAFE. 2022 Dec;247:114249Read more>>
  • Sijie Fan. et al. Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (Citellus dauricus Brandt). INT J MOL SCI. 2022 Jan;23(23):14698Read more>>
  • Chong Li. et al. The adiponectin receptor agonist, AdipoRon, promotes reproductive hormone secretion and gonadal development via the hypothalamic-pituitary-gonadal axis in chickens. POULTRY SCIENCE. 2022 Nov;:102319Read more>>
  • Wenqing Xu. et al. Oral exposure to polystyrene nanoplastics reduced male fertility and even caused male infertility by inducing testicular and sperm toxicities in mice. J HAZARD MATER. 2023 Jul;454:13147Read more>>
  • Heng Yang. et al. miR-665 overexpression inhibits the apoptosis of luteal cells in small ruminants suppressing HPGDS. THERIOGENOLOGY. 2023 Aug;206:4Read more>>
  • Wenjing Lu. et al. Seasonal changes of vitamin D3 and ovarian steroidogenesis in the wild ground squirrels (Citellus dauricus Brandt). J STEROID BIOCHEM. 2023 Nov;234:106385Read more>>
  • Qingjing Gao. et al. Seasonal changes in endoplasmic reticulum stress and steroidogenesis in the ovary of the wild ground squirrels (Citellus dauricus Brandt). GEN COMP ENDOCR. 2023 Nov;343:114368Read more>>
  • Guangxin Yang. et al. Dietary methionine supplementation during the estrous cycle improves follicular development and estrogen synthesis in rats. FOOD FUNCT. 2023 DecRead more>>
  • Wenjing Lu. et al. Vitamin D status alters genes involved in ovarian steroidogenesis in muskrat granulosa cells. BBA-MOL CELL BIOL L. 2024 May;1869:159469Read more>>
  • Yuhang Jia. et al. NTRK2 Promotes Sheep Granulosa Cells Proliferation and Reproductive Hormone Secretion and Activates the PI3K/AKT Pathway. ANIMALS. 2024 Jan;14(10):1465Read more>>
  • Hong Du. et al. Isolation, culture, characterization and the functional study of testicular Leydig cells from Hezuo pig. REPROD DOMEST ANIM. 2024 May;59(5):e14583Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (bs-3906R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (bs-3906R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (bs-3906R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (bs-3906R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.