bs-3907R [Primary Antibody]
Legumain Polyclonal Antibody
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Host: Rabbit

Target Protein: Legumain

Immunogen Range: 201-300/433


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5641

Swiss Prot: Q99538

Source: KLH conjugated synthetic peptide derived from human Legumain

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Flow-Cyt(1ug/Test)

Predicted Molecular Weight: 46


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu Haoge. et al. Number 2 Feibi Recipe Ameliorates Pulmonary Fibrosis by Inducing Autophagy Through the GSK-3_/mTOR Pathway. FRONT PHARMACOL. 2022 Jul;0:2714Read more>>
  • Haoge Liu. et al. Calycosin Ameliorates Bleomycin-Induced Pulmonary Fibrosis via Suppressing Oxidative Stress, Apoptosis, and Enhancing Autophagy. EVID-BASED COMPL ALT. 2022 Oct 11;2022:9969729Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colorectal cancer labeled with Anti Legumain Polyclonal Antibody, Unconjugated (bs-3907R) followed by conjugation to the secondary antibody and DAB staining


Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti Legumain Polyclonal Antibody, Unconjugated (bs-3907R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 49kD. Observed band size: 49kD.


Moues kidney lysates probed with Legumain Polyclonal Antibody, unconjugated (bs-3907R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Mouse placenta lysates probed with Legumain Polyclonal Antibody, unconjugated (bs-3907R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Legumain) Polyclonal Antibody, Unconjugated (bs-3907R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Human JAR cell lysates; Lane 2: Human MCF-7 cell lysates probed with Legumain Polyclonal Antibody, Unconjugated (bs-3907R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.