DATASHEET
Host:
Rabbit
Target Protein:
Legumain
Immunogen Range:
201-300/433
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
5641
Swiss Prot:
Q99538
Source:
KLH conjugated synthetic peptide derived from human Legumain
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
Conjugation:
ALEXA FLUOR® 488
Excitation/ Emission:
490nm/525nm
Size:
100ul
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
46
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Liu Haoge. et al. Number 2 Feibi Recipe Ameliorates Pulmonary Fibrosis by Inducing Autophagy Through the GSK-3_/mTOR Pathway. FRONT PHARMACOL. 2022 Jul;0:2714Read more>>
- Haoge Liu. et al. Calycosin Ameliorates Bleomycin-Induced Pulmonary Fibrosis via Suppressing Oxidative Stress, Apoptosis, and Enhancing Autophagy. EVID-BASED COMPL ALT. 2022 Oct 11;2022:9969729Read more>>
VALIDATION IMAGES
Mouse spleen cells(black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Legumain Polyclonal Antibody, A488 Conjugated (bs-3907R-A488) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).