Host: Rabbit
Target Protein: Lrp2/Megalin
Immunogen Range: 3401-3500/4655
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4036
Swiss Prot: P98164
Source: KLH conjugated synthetic peptide derived from human Megalin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 519
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Jayachandran, Muthuvel, et al. "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." Biol Sex Differ 6.2 (2015).Read more>>
- Erikci, Acelya, Gulberk Ucar, and Samiye Yabanoglu-Ciftci. "Role of serotonin in the regulation of renal proximal tubular epithelial cells." Renal failure 38.7 (2016): 1141-1150.Read more>>
- Giani et al. Renal tubular angiotensin converting enzyme is responsible for nitro-L-arginine methyl ester (L-NAME)-induced salt sensitivity. (2017) Kidney.Int. 91:856-867Read more>>
- Thibodeau JF et al. PBI-4050 via GPR40 activation improves adenine-induced kidney injury in mice. Clin Sci (Lond). 2019 Jul 22;133(14):1587-1602. Read more>>
- Fang-Hao Cai. et al. Diagnostic roles of urinary kidney microvesicles in diabetic nephropathy. Ann Transl Med. 2020 Nov; 8(21): 1431Read more>>
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Megalin Polyclonal Antibody, Unconjugated (bs-3909R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse kidney were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Megalin Polyclonal Antibody(bs-3909R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).