bs-3948R-BF750 [Conjugated Primary Antibody]
LKB1 Polyclonal Antibody, AbBy Fluor® 750 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LKB1

Immunogen Range: 21-120/433


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6794

Swiss Prot: Q15831

Source: KLH conjugated synthetic peptide derived from human LKB1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. In vein endothelial cells, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro). Regulates UV radiation-induced DNA damage response mediated by CDKN1A.

Conjugation: AbBy Fluor® 750

Excitation/ Emission: 749nm/775nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse
Rat
Pig
Sheep

Predicted Cross Reactive Species: Dog
Cow
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology (2016).Read more>>
  • Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.Read more>>
  • Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
  • Yao C et al. Data Mining and Validation of AMPK Pathway as a Novel Candidate Role Affecting Intramuscular Fat Content in Pigs. Animals (Basel). 2019 Apr 1;9(4). pii: E137. Read more>>
  • He L et al. Tumor Suppressor LKB1 inhibits both the mRNA Expression and the Amplification of hTERC by the Phosphorylation of YAP in Lung Cancer Cells. J Cancer 2019; 10(16):3632-3638. Read more>>
  • Zhang LB et al. Pregnancy exposure to carbon black nanoparticles exacerbates bleomycin-induced lung fibrosis in offspring via disrupting LKB1-AMPK-ULK1 axis-mediated autophagy. Toxicology, 2019, 152244. Read more>>
  • Cao Zhanhong. et al. Protective Effects of Huangqi Shengmai Yin on Type 1 Diabetes-Induced Cardiomyopathy by Improving Myocardial Lipid Metabolism. Evid-Based Compl Alt. 2021;2021:5590623Read more>>
  • Chunqiu Fang. et al. Tiaogan Jiejiu Tongluo Formula attenuated alcohol-induced chronic liver injury by regulating lipid metabolism in rats. J ETHNOPHARMACOL. 2023 Dec;317:116838Read more>>
  • Long Chen. et al. Harnessing the potential of postbiotics derived from Bacillus coagulans: A promising avenue for obesity management via the gut-liver axis. FOOD BIOSCI. 2023 Dec;56:103108Read more>>
VALIDATION IMAGES