bs-3955R [Primary Antibody]
MT-ND6 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MT-ND6

Immunogen Range: 51-150/174


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4541

Swiss Prot: P03923

Source: KLH conjugated synthetic peptide derived from human MTND6

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 19


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Sheep
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wu, Ji-hong, et al. "Cumulative mtDNA damage and mutations contribute to the progressive loss of RGCs in a rat model of glaucoma." Neurobiology of Disease (2014).Read more>>
  • Ke Cao. et al. Hypermethylation of Hepatic Mitochondrial ND6 Provokes Systemic Insulin Resistance. 2021 May 02Read more>>
  • Tongling Liufu. et al. Complex I deficiency in m.3243A>G fibroblasts is alleviated by reducing NADH accumulation. FRONT PHYSIOL. 2023; 14: 1164287Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti MT-ND6 Polyclonal Antibody, Unconjugated (bs-3955R) followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MT-ND6 Polyclonal Antibody, Unconjugated (bs-3955R) at 1:400 overnight at 4°C, DAB staining.