bs-3978R [Primary Antibody]
ENO1 Polyclonal Antibody
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Host: Rabbit

Target Protein: ENO1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2023

Source: KLH conjugated synthetic peptide derived from human ENO1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Bovine

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang W et al. Cinnamaldehyde Enhances Antimelanoma Activity through Covalently Binding ENO1 and Exhibits a Promoting Effect with Dacarbazine. Cancers (Basel). 2020 Jan 29;12(2). pii: E311.Read more>>
  • Zhang W et al. Cinnamaldehyde Enhances Antimelanoma Activity through Covalently Binding ENO1 and Exhibits a Promoting Effect with Dacarbazine. Cancers (Basel). 2020 Jan 29;12(2). pii: E311.Read more>>
  • Yihan Lu? et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563.Read more>>
  • Weiyi Zhanget al. Cinnamaldehyde changes the dynamic balance of glucose metabolism by targeting ENO1. Life Sci. 2020 Oct 1;258:118151.Read more>>
  • Sun Wei. et al. The effects of maternal anti-alpha-enolase antibody expression on the brain development in offspring. CLIN EXP IMMUNOL. 2022 SepRead more>>
  • Tianming Lu. et al. Inhibition of ALDOA and ENO1-mediated Glycolysis of Oridonin as a Novel Anti-tumor Strategy of Non-small Cell Lung Cancer. Advanced Therapeutics. 2024 Apr;:2400027Read more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with ENO1 polyclonal antibody, unconjugated (bs-3978R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.