bs-3979R [Primary Antibody]
ENO3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ENO3

Immunogen Range: 341-434/434


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2027

Swiss Prot: P13929

Source: KLH conjugated synthetic peptide derived from human ENO3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

ENO3 is also known as beta enolase, one of the three enolase isoenzymes found in mammals. A switch from alpha enolase to beta enolase occurs in muscle tissue during development and ENO3, a homodimer, is found in skeletal muscle cells in the adult and appears to have a function in striated muscle development and regeneration. Mutations can result in decreased stability of the enzyme and be associated with a glycogen storage myopathy. This results in exercise-induced myalgias, generalized muscle weakness and fatigability.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Flow-Cyt(Flow-Cyt=2ug/Test')

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti ENO3 Polyclonal Antibody (bs-3979R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Pancreas tissue lysates; Lane 4: Rat Liver tissue lysates; Lane 5: Rat Cerebrum tissue lysates; Lane 6: Human HeLa cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human A549 cell lysates; Lane 9: Human Jurkat cell lysates probed with ENO3 Polyclonal Antibody, Unconjugated (bs-3979R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.