DATASHEET
Host:
Rabbit
Target Protein:
ENO3
Immunogen Range:
341-434/434
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
2027
Swiss Prot:
P13929
Source:
KLH conjugated synthetic peptide derived from human ENO3
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
ENO3 is also known as beta enolase, one of the three enolase isoenzymes found in mammals. A switch from alpha enolase to beta enolase occurs in muscle tissue during development and ENO3, a homodimer, is found in skeletal muscle cells in the adult and appears to have a function in striated muscle development and regeneration. Mutations can result in decreased stability of the enzyme and be associated with a glycogen storage myopathy. This results in exercise-induced myalgias, generalized muscle weakness and fatigability.
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti ENO3 Polyclonal Antibody (bs-3979R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Pancreas tissue lysates; Lane 4: Rat Liver tissue lysates; Lane 5: Rat Cerebrum tissue lysates; Lane 6: Human HeLa cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human A549 cell lysates; Lane 9: Human Jurkat cell lysates probed with ENO3 Polyclonal Antibody, Unconjugated (bs-3979R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.