Host: Rabbit
Target Protein: Carbonic Anhydrase 9
Immunogen Range: 251-350/459
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 768
Swiss Prot: Q16790
Source: KLH conjugated synthetic peptide derived from human Carbonic Anhydrase 9
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 46
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Cow
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Gogiraju, Rajinikanth, et al. "Endothelial p53 Deletion Improves Angiogenesis and Prevents Cardiac Fibrosis and Heart Failure Induced by Pressure Overload in Mice." Journal of the American Heart Association 4.2 (2015): e001770.Read more>>
- Jiabao Ling. et al. Designing Lactate Dehydrogenase-Mimicking SnSe Nanosheets To Reprogram Tumor-Associated Macrophages for Potentiation of Photothermal Immunotherapy. ACS APPL MATER INTER. 2022;14(24):2765127665Read more>>
- Qin Wentao. et al. Systematic Construction and Validation of a Novel Ferroptosis-Related Gene Model for Predicting Prognosis in Cervical Cancer. J IMMUNOL RES. 2022;2022:2148215Read more>>
- Jialing Sun. et al. Investigating the molecular mechanism of Qizhu anticancer prescription in inhibiting hepatocellular carcinoma based on high-resolution mass spectrometry and network pharmacology. J ETHNOPHARMACOL. 2024 Feb;:117985Read more>>
- Masuda Shogo. et al. Inhibition of PTPN3 Expressed in Activated Lymphocytes Enhances the Antitumor Effects of Anti-PD-1 Therapy in Head and Neck Cancer, Especially in Hypoxic Environments. J IMMUNOTHER. 2024 Feb;:10.1097/CJI.0000000000000503Read more>>
Lane 1: Rat Stomach lysates probed with Carbonic Anhydrase 9 Polyclonal Antibody, Unconjugated (bs-4029R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Carbonic Anhydrase 9) Polyclonal Antibody, Unconjugated (bs-4029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Carbonic Anhydrase 9) Polyclonal Antibody, Unconjugated (bs-4029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CA9) polyclonal Antibody, Unconjugated (bs-4029R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.