bs-4055R [Primary Antibody]
PPAR alpha (Ser12) Polyclonal Antibody
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]

Host: Rabbit

Target Protein: PPAR alpha Ser12

Modification Site: Ser12

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5465

Swiss Prot: Q07869

Source: KLH conjugated synthetic phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.


Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 52

Cross Reactive Species: Human

Predicted Cross Reactive Species: Dog
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

  • Mölzer, Christine, et al. "Features of an altered AMPK metabolic pathway in Gilbert’s Syndrome, and its role in metabolic health." Scientific Reports 6 (2016): 30051.Read more>>
  • Claudia A. Hana. et al. Serum metabolomics analysis reveals increased lipid catabolism in mildly hyperbilirubinemic Gilbert's syndrome individuals. Metabolism. 2021 Dec;125:154913Read more>>

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti phospho-PPAR alpha(Ser12) Polyclonal Antibody, Unconjugated (bs-4055R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: Rat Liver; 40ug loaded into the lane; Probed with PPAR alpha (Ser12) Polyclonal Antibody, Unconjugated (bs-4055R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Lane 1: Mouse Heart; Lane 2: Mouse Liver; 40ug loaded into the lane; Probed with PAR alpha (Ser12) Polyclonal Antibody, Unconjugated (bs-4055R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

An ELISA was performed using a serial dilution of PPAR Alpha (Ser12) Polyclonal Antibody (bs-4055R) in antigen-coated wells. The antigen used was a peptide containing the phosphosite of interest. The plot shows the absorbance against the antibody dilution for phosphopeptide antigen and non-phosphorylated peptide antigen.

Lane 1: Mouse Heart lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Kidney lysates; Lane 4: Mouse Cerebrum lysates; Lane 5: Rat Cerebrum lysates; Lane 6: Human Jurkat cell lysates; Lane 7: Human A431 cell lysates probed with PPAR alpha Polyclonal Antibody, Unconjugated (bs-4055R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.