bs-4094R [Primary Antibody]
Cdc25B Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cdc25B

Immunogen Range: 501-580/580


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 994

Swiss Prot: P30305

Source: KLH conjugated synthetic peptide derived from human Cdc25B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 65


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse spleen lysates probed with Cdc25B Polyclonal Antibody, Unconjugated (bs-4094R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Mouse Sp2/0 cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human Daudi cell lysates; Lane 6: Human K562 cell lysates probed with Cdc25B Polyclonal Antibody, Unconjugated (bs-4094R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.