bs-41218R-BF488 [Conjugated Primary Antibody]
Galectin 3 Polyclonal Antibody, AbBy Fluor-488 Conjugated
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Galectin 3

Immunogen Range: 1-250/250


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3958

Swiss Prot: P17931

Source: Recombinant human Galectin-3 protein

Purification: affinity Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

Conjugation: AbBy Fluor-488

Excitation/ Emission: 499nm/519nm

Size: 100uL

Concentration: 1mg/ml

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)

Predicted Molecular Weight: 29


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: LGALS3 | MAC2 Antibody, conjugated (bs-41218R-BF488) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: LGALS3 | MAC2 Antibody, conjugated (bs-41218R-BF488) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: LGALS3 | MAC2 Antibody, conjugated (bs-41218R-BF488) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: LGALS3 | MAC2 Antibody, conjugated (bs-41218R-BF488) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: LGALS3 | MAC2 Antibody, conjugated (bs-41218R-BF488) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: LGALS3 | MAC2 Antibody, conjugated (bs-41218R-BF488) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.