bs-41230P
[Protein]
Tags Positive Control Whole Cell Lysate
DATASHEET
Swiss Prot: N/A
Source: Tags Positive Control Whole Cell Lysate protein is expressed in E.coli with His. It contains the amino acid sequence of .
Storage Buffer: 0.01M TBS(pH7.4)
Storage: The product should be stored at -70°C or -20°C.
Background:
This E.coli lysate contains a 41 kd recombinant protein expressing 11 commonly used Tags which are fused to the N-terminus of the protein in the order of:
T7 HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
GST
Tag sequences:
T7 = CMASMTGGQQMG
HSV = QPELAPEDPEDC
CMYC = EQKLISEEDLC
VSV-g = YTDIEMNRLGK
Glu-Glu = EYMPME
V5 = GKPIPNPLLGLDST
E-tag = GAPVPYPDPLEPR
FLAG = DYKDDDDK
HA = YPYDVPDYAC
6xHIS = HHHHHH
Lyophilised: After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and then stored at –20°C. The sample can be reused several times.
Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.
T7 HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
GST
Tag sequences:
T7 = CMASMTGGQQMG
HSV = QPELAPEDPEDC
CMYC = EQKLISEEDLC
VSV-g = YTDIEMNRLGK
Glu-Glu = EYMPME
V5 = GKPIPNPLLGLDST
E-tag = GAPVPYPDPLEPR
FLAG = DYKDDDDK
HA = YPYDVPDYAC
6xHIS = HHHHHH
Lyophilised: After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and then stored at –20°C. The sample can be reused several times.
Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.
Size: 250ug
Applications: ELISA()
Mol. Wt.: 41
Species: Others
For research use only. Not intended for diagnostic or therapeutic use.