bs-41239R [Primary Antibody]
Lamin A/C Polyclonal Antibody
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Host: Rabbit

Target Protein: Lamin A/C

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4000

Swiss Prot: P02545

Source: Recombinant human lamin A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Play an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 73


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human U87MG cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human Hela cell lysates probed with Lamin A/C Polyclonal Antibody, Unconjugated (bs-41239R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with Lamin A/C polyclonal antibody, unconjugated (bs-41239R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.